Spatial Sequencing Analysis

10× Genomics Visium Spatial Transcriptomics

The BIG Core provides 10× Genomics Visium Spatial transcriptomics analysis for fresh frozen or FFPE samples. With Visium CytAssist, tissue sectioning, staining (H&E or immunofluorescence), and imaging take place on a standard glass slide, followed by hybridization of transcriptomic probes to a Visium slide with either a 6.5 × 6.5 or 11×11 mm Visium Capture Area. After probe extension and library construction following the standard Visium workflow, the pooled libraries are then sequenced on Illumina NextSeq 2000.