Short-Read Sequencing
The MiSeq system offers the first end-to-end sequencing solution, integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. The MiSeq is best suited for small RNA sequencing, 16S metagenomics, amplicon sequencing, small to medium targeted panels, whole-genome sequencing of smaller model organism genomes, quality control of libraries, and more. Flow cells are available in 3 sizes: Nano (up to 1M reads), Micro (up to 4M reads), and Regular (up to 15M with v2 reagent and 25M with v3 reagent). Reagents are available for different read lengths: 50, 300, and 500 cycles for v2 reagent; 150 and 600 cycles for v3 reagent. Both single- and paired-end sequencing can be used, with the maximum read length at 300 bp.
The NextSeq 2000 system with DRAGEN Bio-IT platform integration offers the newest end-to-end sequencing solution, providing sequencing power for high-throughput applications and computing power for ultra-rapid sequencing data analyses. The NextSeq 2000 supports single-cell gene expression (10 samples with P2 reagent, 4k cells/sample, 10k reads/cell), whole-exome sequencing (16 samples with P2 reagent, 50× coverage), whole-genome sequencing (3 human samples with P3 reagent, 30× coverage), and shotgun metagenomics (8 samples with P2 reagent, 50M reads/sample).
